Modification History
Not applicable.
Unit Descriptor
Unit descriptor |
This unit of competency covers the ability to contribute to the culture, isolation and identification of micro-organisms, such as bacteria, fungi, viruses, protozoans, algae and parasites in order to investigate the physiology and pathology of plants and animals, monitor the natural environment, and to assist in the production of foods, pharmaceutical goods and other manufactured materials. |
Application of the Unit
Application of the unit |
This unit of competency is applicable to laboratory technicians and technical officers working in the biomedical, biotechnology, environmental, manufacturing and food processing industry sectors. The results of work performed by technical personnel would normally be integrated, interpreted and reported on by scientists, medical, veterinary or plant pathologists or other responsible officers of an enterprise. Although a supervisor may not always be present, the technician will follow standard operating procedures (SOPs) that will clearly describe the scope of permitted practice in modifying testing procedures, interpreting of data and for communicating test results to people outside the laboratory. It is applicable to investigations of as well as addressing the broader needs of biotechnology and tissue culture applications. Industry representatives have provided case studies to illustrate the practical application of this unit of competency and to show its relevance in a workplace setting. These can be found at the end of this unit of competency under the section 'This competency in practice'. |
Licensing/Regulatory Information
Not applicable.
Pre-Requisites
Prerequisite units |
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MSL974006A |
Perform biological procedures |
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MSL973007A |
Perform microscopic examination |
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MSL973004A |
Perform aseptic techniques |
Employability Skills Information
Employability skills |
This unit contains employability skills. |
Elements and Performance Criteria Pre-Content
Elements describe the essential outcomes of a unit of competency. |
Performance criteria describe the performance needed to demonstrate achievement of the element. Where bold italicised text is used, further information is detailed in the required skills and knowledge section and the range statement. Assessment of performance is to be consistent with the evidence guide. |
Elements and Performance Criteria
ELEMENT |
PERFORMANCE CRITERIA |
1. Receive samples and process associated request forms |
1.1. Check samples and request form details before they are accepted 1.2. Return samples and request forms that do not comply with requirements to source with reasons for non-acceptance 1.3. Log samples, recording details that allow accurate tracking and chain of custody 1.4. Distribute samples for local testing or dispatch samples to other testing facilities 1.5. Store samples appropriately where testing or transport is to be delayed |
2. Prepare for safe microbiological work and aseptic applications |
2.1. Select work area and equipment required for the safe handling of materials that may contain micro-organisms of specified risk groups 2.2. Wear protective apparel, replacing it when contamination is suspected 2.3. Apply correct disinfection procedures to work areas before and after use 2.4. Locate relevant emergency equipment for timely response to microbiological accidents 2.5. Apply standard precautions when handling biological materials 2.6. Minimise the production and release of aerosols, using biological safety cabinets where necessary 2.7. Clean spills, and report all spills and suspected incidents to supervisor 2.8. Wash hands before and after laboratory work and when contamination is suspected 2.9. Ensure the safe disposal of biohazardous materials and other laboratory wastes in accordance with enterprise procedures |
3. Process samples for direct examination |
3.1. Prepare thin smears of samples for subsequent staining to enable microscopic identification of cells 3.2. Prepare liquid films of specimens for direct observation for motility or cell structure 3.3. Prepare samples to concentrate material for subsequent staining or microscopy |
4. Prepare pure cultures for microbiological work and aseptic applications |
4.1. Select culture media to maximise growth of micro-organisms and cells 4.2. Inoculate media aseptically, applying techniques suitable for purpose of culture 4.3. Incubate inoculated media in conditions to optimise growth of organisms and cells 4.4. Subculture on suitable media to optimise production of pure cultures |
5. Perform procedures that can assist in the identification of micro-organisms |
5.1. Select staining techniques to demonstrate required cellular characteristics 5.2. Stain prepared films to demonstrate diagnostically useful characteristics 5.3. Inoculate and incubate media with pure cultures to assist in the biochemical and immunological identification of micro-organisms 5.4. Perform tests on pure cultures to assist in the biochemical and immunological identification of micro-organisms |
6. Estimate the number and/or size of micro-organisms in samples |
6.1. Count cells in undiluted samples to indicate the dilution necessary to reliably count organisms in culture 6.2. Prepare serial dilutions of samples aseptically for culture and colony counting 6.3. Count colonies for calculating number of viable organisms per unit volume 6.4. Count micro-organisms in samples and cultures using spectrometric and electronic methodologies, where relevant 6.5. Estimate and document uncertainty of measurement in accordance with enterprise procedures, where relevant |
7. Contribute to antibiotic sensitivity testing where required |
7.1. Prepare inoculum suitable for antibiotic sensitivity testing 7.2. Dispense or position antibiotic discs as indicated by enterprise protocol 7.3. Incubate inoculated media under conditions to maximise growth of cultured organism 7.4. Read and record sensitivity reactions, noting phenomena that can assist in the correct interpretation of results |
8. Maintain records of laboratory work |
8.1. Make entries on report forms or into computer systems, accurately calculating, recording or transcribing data as required 8.2. Maintain instrument logs as required by accreditation checklists 8.3. Maintain security and confidentiality of all clinical information, laboratory data and records |
Required Skills and Knowledge
REQUIRED SKILLS AND KNOWLEDGE |
This section describes the skills and knowledge required for this unit. |
Required skills |
Required skills include:
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Required knowledge |
Required knowledge includes:
Specific industry Additional knowledge requirements may apply for different industry sectors. For example: Biomedical and biotechnology:
Biological and environmental:
Food processing:
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Evidence Guide
EVIDENCE GUIDE |
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The Evidence Guide provides advice on assessment and must be read in conjunction with the performance criteria, required skills and knowledge, range statement and the Assessment Guidelines for the Training Package. |
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Overview of assessment |
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Critical aspects for assessment and evidence required to demonstrate competency in this unit |
Assessors should ensure that candidates can:
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Context of and specific resources for assessment |
This unit of competency is to be assessed in the workplace or simulated workplace environment. This unit of competency may be assessed with:
Resources may include:
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Method of assessment |
The following assessment methods are suggested:
In all cases, practical assessment should be supported by questions to assess underpinning knowledge and those aspects of competency which are difficult to assess directly. Where applicable, reasonable adjustment must be made to work environments and training situations to accommodate ethnicity, age, gender, demographics and disability. Access must be provided to appropriate learning and/or assessment support when required. The language, literacy and numeracy demands of assessment should not be greater than those required to undertake the unit of competency in a work like environment. |
This competency in practice |
Industry representatives have provided the case studies below to illustrate the practical application of this unit of competency and to show its relevance in a workplace setting. Biomedical A patient's urine sample and request form have been brought to the laboratory for urgent testing. After preparation of the work area, the technical officer examines a cover-slipped preparation of the sample and notes the presence of pus cells and non-motile rod organisms. In a Gram stain he confirms the presence of pus cells and Gram negative bacilli. They inoculate a MacConkey's and a blood agar plate for growth and isolation of bacteria. After consultation with the supervisor they are asked to set up a direct culture for antibiotic sensitivity testing. The supervisor informs the clinician of the initial findings. The next morning the technical officer assists the supervisor to read the plates. The predominance of lactose fermenting organisms is noted. The supervisor asks the technical officer to set up a biochemical panel to assist in identifying the organism. The supervisor confirms the technical officer's reading of the direct sensitivities plate. Later in the day the team is able to confirm that the patient's urine is infected with Escherichia coli and that the organism is sensitive to a number of antibiotics, including a sulphonamide and a cephalosporin. Food processing A swollen can of tuna was received at the company laboratory for microbiological investigation. The technical officer recorded the details supplied with the can and prepared for the investigation. A range of media, including cooked meat media and nutrient broth were prepared and aseptic can opening equipment was sterilised. After the can was opened in the biohazard cabinet, the state of the contents was recorded, pH checked and Gram stains prepared and examined. The media was inoculated with the food samples and incubated at a range of temperatures under aerobic and anaerobic conditions. The can was then emptied for double seam tear down to determine the cause of the spoilage. The next day the technical officer examined the media and broth cultures. From all the data collected the technical officer and supervisor were able to determine that pre-processing spoilage had occurred, probably due to excessive delays in the process prior to can sterilisation. The results were reported to production personnel so that they could follow up the circumstances relating to the delays, and ensure that the SOP had been followed and sufficient product rejected. |
Range Statement
RANGE STATEMENT |
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The range statement relates to the unit of competency as a whole. It allows for different work environments and situations that may affect performance. Bold italicised wording, if used in the performance criteria, is detailed below. Essential operating conditions that may be present with training and assessment (depending on the work situation, needs of the candidate, accessibility of the item, and local industry and regional contexts) may also be included. |
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Codes of practice |
Where reference is made to industry codes of practice, and/or Australian/international standards, it is expected the latest version will be used |
Standards , codes , procedures and /or enterprise requirements |
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Equipment , materials and systems |
Equipment, materials and systems may include:
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Communication |
Communication may involve:
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Occupational health and safety (OHS ) and environmental management requirements |
OHS and environmental management requirements:
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Unit Sector(s)
Unit sector |
Testing |
Competency field
Competency field |
Co-requisite units
Co-requisite units |
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