Modification History
Not applicable.
Unit Descriptor
Unit descriptor |
This unit of competency covers the ability to analyse samples using advanced gas chromatography (GC) instruments including GC-MS. The unit also includes establishing client needs for routine and non-routine samples, optimising enterprise procedures and instruments for specific samples, obtaining valid and reliable data and reporting test results. Personnel are required to recognise atypical test data/results and troubleshoot common analytical instrument and procedure problems and perform routine instrument maintenance. |
Application of the Unit
Application of the unit |
This unit of competency is applicable to experienced laboratory technical officers/technicians, laboratory supervisors and technical specialists who conduct instrumental analysis in laboratories providing consultancy, research and development and quality assurance services. These services may be provided for a wide range of industry sectors, such as forensic science (e.g. fire debris, toxicology and explosives), biomedical (e.g. isotopic labelling of metabolites), environmental monitoring (e.g. organics), food and beverage processing (e.g. aromatics, pesticide residues) and pharmaceuticals (e.g. active ingredient) testing. Industry representatives have provided case studies to illustrate the practical application of this unit of competency and to show its relevance in a workplace setting, at the end of this unit of competency under the section 'This competency in practice'. |
Licensing/Regulatory Information
Not applicable.
Pre-Requisites
Prerequisite units |
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Employability Skills Information
Employability skills |
This unit contains employability skills. |
Elements and Performance Criteria Pre-Content
Elements describe the essential outcomes of a unit of competency. |
Performance criteria describe the performance needed to demonstrate achievement of the element. Where bold italicised text is used, further information is detailed in the required skills and knowledge section and the range statement. Assessment of performance is to be consistent with the evidence guide. |
Elements and Performance Criteria
ELEMENT |
PERFORMANCE CRITERIA |
1. Determine sample characteristics and appropriate analytical methods |
1.1. Interpret client request and/or identify sample characteristics that may affect sample preparation and/or analysis 1.2. Liaise with client or sample provider to review client needs, testing requirements and sample history. if necessary 1.3. Identify analytical standards, reference materials, test methods and enterprise procedures that may be applicable 1.4. Select the most appropriate standard test method that is consistent with testing requirements and instrument availability 1.5. If no standard method exists, adapt or modify a test method to suit the sample characteristics 1.6. If necessary, seek advice from supervisor about any proposed variations and document all approved changes to test methods 1.7. Schedule analysis using enterprise procedures |
2. Prepare samples and standards |
2.1. Log sample into instrument software 2.2. Obtain a representative analytical portion of the laboratory sample 2.3. Prepare sample in accordance with selected test method 2.4. Prepare validation checks and/or calibration standards for analytical portions 2.5. Use specialised procedures for ultra trace sample and standard preparation as required |
3. Set up instrument and perform trial analysis |
3.1. Configure the gas flow, injector, column, oven and detector sub-systems according to the selected test method 3.2. Perform pre-use, calibration and safety checks using enterprise procedures 3.3. Set instrumental parameters in accordance with those specified in selected test method 3.4. Check and optimise each instrument sub-system 3.5. Select an appropriate internal standard, if required 3.6. Conduct performance tests using (internal standards), standards and samples 3.7. Assess instrument performance in terms of response, resolution and run-time |
4. Optimise instrument performance |
4.1. Apply an understanding of analyte and column chemistry, temperature control and gas flow rate to determine strategies for enhancing separation and detection of required species 4.2. Adjust instrumental parameters in a logical and efficient sequence to optimise performance 4.3. When optimum separation is achieved, check that the detector and system software can correctly identify and quantify the required species |
5. Perform analysis |
5.1. Measure analyte (and internal standard) response for standards, validation checks and samples using optimised instrument settings 5.2. Conduct sufficient measurements to obtain reliable data 5.3. Regularly check for calibration drift and take appropriate action as necessary 5.4. Use system software to produce calibration graphs, chromatographs and/or mass spectra, confirm data quality and calculate uncertainties 5.5. Check that results are consistent with estimations and expectations 5.6. Analyse trends in data and/or results and report out of specification or atypical results promptly to appropriate personnel 5.7. Return instrument to standby or shutdown condition in accordance with enterprise procedures 5.8. Report results with the appropriate accuracy, precision, uncertainty and units |
6. Perform routine maintenance and troubleshoot instruments |
6.1. Regularly check the condition of gas cylinders, filters and traps and replace as necessary 6.2. Regularly check that the injector, column and detector sub-systems are clean/undamaged and replace consumable items as necessary 6.3. Change columns in accordance with manufacturer's instructions and ensure that the system is free of leaks and properly conditioned before re-use 6.4. Investigate possible causes for the absence of peaks and presence of ghost peaks, split peaks or distorted peak shapes and apply recommended remedial actions 6.5. Investigate possible causes for baseline instability and non-reproducible retention times and apply recommended remedial actions 6.6. Identify the need for repairs or servicing and determine whether local repair/maintenance is technically possible and economic 6.7. Arrange for repair or servicing from an accredited agent or other appropriate personnel in accordance with enterprise procedures |
7. Maintain a safe work environment |
7.1. Identify risks, hazards, safety equipment and control measures associated with sample handling/preparation and test method 7.2. Use personal protective equipment and safety procedures specified for test method and materials to be tested 7.3. Minimise the generation of wastes and environmental impacts 7.4. Ensure the safe collection/disposal of laboratory wastes 7.5. Clean, care for and store equipment and consumables in accordance with enterprise procedures |
8. Maintain laboratory records |
8.1. Enter approved data and results into laboratory information management system (LIMS) 8.2. Maintain logs of instrument calibration checks, use and maintenance in accordance with enterprise procedures 8.3. Maintain security, integrity and traceability of samples, results and documentation 8.4. Communicate results to appropriate personnel in accordance with enterprise procedures |
Required Skills and Knowledge
REQUIRED SKILLS AND KNOWLEDGE |
This section describes the skills and knowledge required for this unit. |
Required skills |
Required skills include:
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Required knowledge |
Required knowledge includes:
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Evidence Guide
EVIDENCE GUIDE |
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The Evidence Guide provides advice on assessment and must be read in conjunction with the performance criteria, required skills and knowledge, range statement and the Assessment Guidelines for the Training Package. |
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Overview of assessment |
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Critical aspects for assessment and evidence required to demonstrate competency in this unit |
Assessors should ensure that candidates can:
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Context of and specific resources for assessment |
This unit of competency is to be assessed in the workplace or simulated workplace environment. This unit of competency may be assessed with:
Resources may include:
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Method of assessment |
The following assessment methods are suggested:
In all cases, practical assessment should be supported by questions to assess underpinning knowledge and those aspects of competency which are difficult to assess directly. Where applicable, reasonable adjustment must be made to work environments and training situations to accommodate ethnicity, age, gender, demographics and disability. Access must be provided to appropriate learning and/or assessment support when required. The language, literacy and numeracy demands of assessment should not be greater than those required to undertake the unit of competency in a work like environment. |
This competency in practice |
Industry representatives have provided the case studies below to illustrate the practical application of this unit of competency and to show its relevance in a workplace setting. Environmental testing A laboratory routinely analyses samples of foodstuffs for pesticide residues. Traditionally, they have used GC combined with ECD and FID detectors to quantify one or two classes at a time. Recently, the laboratory has commissioned a new GC-MS instrument that is capable of quantifying low level pesticides using the SIM mode while simultaneously performing quantification of higher concentrations using full-scan (SIFI single ion and full ion) acquisition. The technician sets up for a typical run of samples. He/she uses the programmable split/splitless injector to provide 1µL samples. The injection port temperature is set at 275°C (isothermal) and the capillary column uses a phase specifically designed for separation of pesticides. The helium carrier gas is programmed with a constant velocity of 30 cm/s. The oven temperature program is initially set to 80°C with no hold and ramped to 290°C at 20°C/min with a hold of 4.5 min. The total oven program is 15 min, with an injection to injection time of less than 20 min. The MS method contains multiple SIM functions overlapped by a m /z 40 to m /z 450 full scan function and the mass spectrometer transfer line and ion source are heated to 275°C. Pharmaceutical testing A laboratory technician working for a major cosmetics company is given a retail sample of a competitor's perfume and asked to determine its composition. He/she has to start somewhere and decides to use one of the laboratory's GC-MS instruments to simplify the identification process and to avoid the need for multiple spikes. The GC-MS is already set up to measure the company's own perfume and so the technician uses standard instrumental parameters as a starting point. However, he/she finds that many peaks are not resolved and some take a long time to elute. The technician realises that he/she should either modify the temperature and/or pressure programming or change columns. Sensibly, the technician chooses to modify the easiest parameter (i.e. temperature) before changing the column. This overcomes the problem of slow elution but does not resolve all peaks. He/she then varies the pressure programming which provides better resolution but does not completely separate all peaks. The technician then uses the instrument's spectral matching software to identify as many peaks as possible, including the ones that overlap. |
Range Statement
RANGE STATEMENT |
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The range statement relates to the unit of competency as a whole. It allows for different work environments and situations that may affect performance. Bold italicised wording, if used in the performance criteria, is detailed below. Essential operating conditions that may be present with training and assessment (depending on the work situation, needs of the candidate, accessibility of the item, and local industry and regional contexts) may also be included. |
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Codes of practice |
Where reference is made to industry codes of practice, and/or Australian/international standards, it is expected the latest version will be used |
Standards , codes , procedures and /or enterprise requirements |
Standards, codes, procedures and/or enterprise requirements may include:
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GC instruments and techniques |
GC instruments and techniques may include:
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Testing that uses GC instruments |
Testing that uses GC instruments may include:
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Sample characteristics that may affect analysis |
Sample characteristics that may affect analysis may include:
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Sample preparation |
Sample preparation may include:
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Instrumental parameters |
Instrumental parameters may include
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Common analytical procedure and equipment problems |
Common analytical procedure and equipment problems may include:
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Hazards |
Hazards may include:
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Addressing hazards |
Addressing hazards may include:
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Occupational health and safety (OHS ) and environmental management requirements |
OHS and environmental management requirements:
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Unit Sector(s)
Unit sector |
Testing |
Competency field
Competency field |
Co-requisite units
Co-requisite units |
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