Modification History
Not applicable.
Unit Descriptor
Unit descriptor |
This unit of competency covers the ability to analyse samples using electrophoretic techniques. The unit also includes establishing client needs for routine and non-routine samples, optimising enterprise procedures and instruments for specific samples, obtaining valid and reliable data and reporting test results. Personnel are required to recognise atypical test data/results and troubleshoot common analytical procedure and equipment problems. |
Application of the Unit
Application of the unit |
This unit of competency is applicable to laboratory technical officers working in all industry sectors. All operations and analytical methods must comply with relevant standards, appropriate procedures and/or enterprise requirements. Although a supervisor may not always be present, the technician will follow standard operating procedures (SOPs) that clearly describe their scope of permitted practice, including varying enterprise/test procedures and communicating results to people outside the laboratory. Industry representatives have provided case studies to illustrate the practical application of this unit of competency and to show its relevance in a workplace setting. These can be found at the end of this unit of competency under the section 'This competency in practice'. |
Licensing/Regulatory Information
Not applicable.
Pre-Requisites
Prerequisite units |
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MSL973002A |
Prepare working solutions |
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OR |
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MSL974001A |
Prepare , standardise and use solutions |
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MSL974003A |
Perform chemical tests and procedures |
Employability Skills Information
Employability skills |
This unit contains employability skills. |
Elements and Performance Criteria Pre-Content
Elements describe the essential outcomes of a unit of competency. |
Performance criteria describe the performance needed to demonstrate achievement of the element. Where bold italicised text is used, further information is detailed in the required skills and knowledge section and the range statement. Assessment of performance is to be consistent with the evidence guide. |
Elements and Performance Criteria
ELEMENT |
PERFORMANCE CRITERIA |
1. Establish client needs and schedule analysis |
1.1. Liaise with client or sample provider to determine client needs and sample history 1.2. Record sample description, compare with specification and record and report discrepancies 1.3. Identify non-routine samples and the possible need to vary enterprise procedures 1.4. Seek advice from supervisor about any proposed variations and document all approved changes 1.5. Schedule analysis using enterprise procedures |
2. Prepare samples and standards |
2.1. Obtain a representative analytical portion of the laboratory sample 2.2. Prepare sample in accordance with testing requirements 2.3. Prepare validation checks for analytical portion |
3. Set up and optimise instrument |
3.1. Perform pre-use and safety checks in accordance with enterprise procedures 3.2. Start up and condition the instrument using enterprise procedures 3.3. Optimise instrumental parameters to suit sample and test requirements 3.4. Check calibration status of instrument and perform calibration using specified standards and procedures, if applicable |
4. Perform analysis |
4.1. Measure analyte response for standards, validation checks and samples 4.2. Conduct sufficient measurements to obtain reliable data 4.3. Return instruments to standby or shutdown condition as required |
5. Process and analyse data |
5.1. Confirm data is the result of valid measurements 5.2. Perform required calculations and ensure results are consistent with standards or estimations and expectations 5.3. Record results with the appropriate accuracy, precision and units 5.4. Analyse trends in data and/or results and report out of specification or atypical results promptly to appropriate personnel 5.5. Troubleshoot analytical procedure or equipment problems which have led to atypical data or results |
6. Maintain a safe work environment |
6.1. Identify risks, hazards, safety equipment and control measures associated with sample handling, preparation and analytical method 6.2. Use personal protective equipment and safety procedures specified for test method and materials to be tested 6.3. Minimise the generation of wastes and environmental impacts 6.4. Ensure the safe disposal of laboratory wastes 6.5. Clean, care for and store equipment and consumables in accordance with enterprise procedures |
7. Maintain laboratory records |
7.1. Enter approved data and results into laboratory information management system 7.2. Maintain equipment logs in accordance with enterprise procedures 7.3. Maintain security, integrity and traceability of samples and documentation 7.4. Communicate results to appropriate personnel |
Required Skills and Knowledge
REQUIRED SKILLS AND KNOWLEDGE |
This section describes the skills and knowledge required for this unit. |
Required skills |
Required skills include:
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Required knowledge |
Required knowledge includes:
Specific industry Additional knowledge requirements may apply for different industry sectors. For example: Biomedical and environmental services:
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Evidence Guide
EVIDENCE GUIDE |
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The Evidence Guide provides advice on assessment and must be read in conjunction with the performance criteria, required skills and knowledge, range statement and the Assessment Guidelines for the Training Package. |
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Overview of assessment |
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Critical aspects for assessment and evidence required to demonstrate competency in this unit |
Assessors should ensure that candidates can:
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Context of and specific resources for assessment |
This unit of competency is to be assessed in the workplace or simulated workplace environment. This unit of competency may be assessed with:
Resources may include:
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Method of assessment |
The following assessment methods are suggested:
In all cases, practical assessment should be supported by questions to assess underpinning knowledge and those aspects of competency which are difficult to assess directly. Where applicable, reasonable adjustment must be made to work environments and training situations to accommodate ethnicity, age, gender, demographics and disability. Access must be provided to appropriate learning and/or assessment support when required. The language, literacy and numeracy demands of assessment should not be greater than those required to undertake the unit of competency in a work like environment. |
This competency in practice |
Industry representatives have provided the case studies below to illustrate the practical application of this unit of competency and to show its relevance in a workplace setting. Environmental The advent of DNA typing in the mid-1980s has enormously increased the ability of forensic technicians to identify individuals uniquely by testing a variety of their body fluids found at the crime scene. The samples obtained from the scene are first treated to extract the DNA with short tandem repeated (STR) markers. After isolating the DNA from its cells, specific regions are copied by the polymerase chain reaction (PCR). The resulting PCR products are then separated and detected in order to characterise the STR region being examined. The most common separation methods used today are slab gel and capillary electrophoresis (CE). Given the enormous number of DNA samples to be processed, technicians frequently run fully automated injection, separation and detection stops. They use computerised data acquisition to enable rapid analysis and subsequent searching of digital storage of DNA results. Food processing Technicians who work in the food and beverage processing industries regularly monitor the purity of food additives, such as dyes and colouring agents in products, such as sweets and soft drinks. For example, technicians may sample a batch of soft drink by low temperature evaporation of a known percentage of the water and then subject the remains to electrophoresis separation technique. In this way, both the identification and concentration of a dye (or other additive) present in the soft drink can be determined. Technicians may also be required to examine the electrophoresis results for any indication of harmful or toxic impurities, which may have inadvertently contaminated the product. Quality control and use of appropriate standards are important components of these analytical procedures. |
Range Statement
RANGE STATEMENT |
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The range statement relates to the unit of competency as a whole. It allows for different work environments and situations that may affect performance. Bold italicised wording, if used in the performance criteria, is detailed below. Essential operating conditions that may be present with training and assessment (depending on the work situation, needs of the candidate, accessibility of the item, and local industry and regional contexts) may also be included. |
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Codes of practice |
Where reference is made to industry codes of practice, and/or Australian/international standards, it is expected the latest version will be used |
Standards , codes , procedures and /or enterprise requirements may include : |
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Electrophoretic methods , including both analytical and preparative procedures |
Electrophoretic methods, including both analytical and preparative procedures, may use:
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Preparation of sample |
Preparation of sample may include pre-treatment processes, such as:
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Tests |
Tests may include methods for:
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Common analytical procedure and equipment problems |
Common analytical procedure and equipment problems may include:
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Hazards |
Hazards may include:
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Addressing hazards |
Addressing hazards may involve:
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Occupational health and safety (OHS ) and environmental management requirements |
OHS and environmental management requirements:
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Unit Sector(s)
Unit sector |
Testing |
Competency field
Competency field |
Co-requisite units
Co-requisite units |
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